Diler U S Altay
Ordu University, Turkey
Title: Investigation of piR-36707 and piR-36741 expression levels in renal cell (transparent cell type) carcinomas
Biography
Biography: Diler U S Altay
Abstract
Background: Piwi interacting RNA (piRNA) is the main class of small non-coding RNA molecules expressed in animal cells. Some of the piRNAs expression increases and shows oncogenic properties; while on the other hand, some of their expression decreases and shows tumorigenic properties in specific cancer types.
Objective: The present research is in comparison within normal and tumor renal biopsy specimens taken from patients with clear cell renal cell carcinoma (RCC), and in kidney tissue samples of non-transfected RCC patients who were operated for different reasons, two possible piRNAs (piR -36707 and piR-36741) expression levels, which are not related before but probably related to RCC.
Methodology & Theoretical Orientation: Between January 2016 and January 2017, formalin-fixed paraffin embedded (FFPE) specimens were obtained from 19 patients who had undergone partial or radical nephrectomy for diagnostic purposes and who had a clear cell RCC diagnosis in urology and pathology in the Ondokuz Mayıs University, Faculty of Medicine (OMÜTF). Tumor tissue sample and a healthy tissue sample from the same patient were included in the study. FFPE was made from tissue using a total RNA isolation kit. The piRNA-specific cDNA synthesis kit was used to convert the resulting piRNA into cDNA. piR-36707 and piR-36741 expression levels were measured using commercially available primers and real-time PCR kit specific to these piRNAs. Statistical significance analysis of the levels of piRNA expression was performed between the two groups.
Findings: Expression levels of piR-36707 and piR-36741, calculated by the comparative 2-ΔΔCt curve between tumor and normal kidney tissues, are given in comparison in Figure 1. The higher expression levels of piR-36707 and piR-36741 in tumor tissues than normal tissues give them a potential oncogenic function. However, when this relationship was examined by statistical methods, it was seen that p value was above 0.05 for both piRNAs and these changes were not significant.
Conclusion & Significance: According to the literature, this is the first study that relates to clear cell RCC pathogenesis and piR-36707 and piR-36741 genes. Prospectively, all genes in the target of piR-36707 and piR-36741 are studied as panels to achieve universal and comprehensive data for the pathogenesis of clear cell RCC.