Manar Atoum
Hashemite university, Jordan
Title: Polymorphisms of leptin (-2548 G/A) and leptin receptor (Q223R) genes among jordanian breast cancer females
Biography
Biography: Manar Atoum
Abstract
Background: Breast cancer is the most common cancer among females. Breast cancer is curable during its early stages and is lethal during its late stages.
Aims: This study is aimed to assess the possible association of leptin levels with breast cancer risk, in addition to investigate the relationship between LEP (-2548 G/A) and LEPR (Q223R) polymorphism and breast cancer risk among Jordanian females.
Methods: A total of 60 blood samples collected from breast cancer patients were analyzed to identify; the serum level of leptin and leptin receptor related polymorphism. A total of 40 healthy females were enrolled as control group. Serum level of leptin was detected by enzyme-linked immunosorbent assay (ELISA), DNA from patients and control were amplified and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
Results: The distribution of the two polymorphisms in the studied population was consistent with the Hardy-Weinberg equilibrium. Leptin level was significantly lower in breast cancer patients compared to the controls (p=0.05).
Conclusion: Our results showed a significant association between low leptin levels in serum and Q223R polymorphism(in leptin receptor gene) with an increase of breast cancer risk. Moreover, no association was found between -2548 G/A polymorphism (in leptin gene) with breast cancer risk. Further studies with larger sample size are suggested.